International Science Index


Mycoflora of Activated Sludge with MBRs in Berlin, Germany

Abstract:Thirty six samples from each (aerobic and anoxic) activated sludge were collected from two wastewater treatment plants with MBRs in Berlin, Germany. The samples were prepared for count and definition of fungal isolates; these isolates were purified by conventional techniques and identified by microscopic examination. Sixty tow species belonging to 28 genera were isolated from activated sludge samples under aerobic conditions (28 genera and 58 species) and anoxic conditions (26 genera and 52 species). The obtained data show that, Aspergillus was found at 94.4% followed by Penicillium 61.1 %, Fusarium (61.1 %), Trichoderma (44.4 %) and Geotrichum candidum (41.6 %) species were the most prevalent in all activated sludge samples. The study confirmed that fungi can thrive in activated sludge and sporulation, but isolated in different numbers depending on the effect of aeration system. Some fungal species in our study are saprophytic, and other a pathogenic to plants and animals.
[1] K. Ulfig, "Studies of keratinolytic and keratinophilic fungi in sewage sludge by means of a multi-temperature hair baiting method," Polish Journal of Environmental Studies, vol. 12, no. 4, pp.461-466. 2003.
[2] K. Ulfig, "Sludge liming decreases the growth of keratinolytic and Keratinophilic fungi," Polish Journal of Environmental Studies, vol. 15, no. 2, pp. 341-346. 2006.
[3] M. Kacperzak, E. Neczaj, and E. Okoniewska, "The comparative mycological analysis of wastewater and sewage sludge from selected wastewater treatment plants, Desalination" vol. 185, no. 1-3, pp. 363- 370. 2005.
[4] I. Rosas, A. Baez, and M. Coutino, "Bacteriological Quality of Crops Irrigation with Wastewater in the Xochimilco plots, Mexico City, Mexico," Applid and Environ Microbio. May, pp. 1074-1079. 1984.
[5] A. H. S. Al-Zubeiry, "Microflora inhabiting raw sewage, secondary effluent and dewatered sludge in IBB, YEMEN REPUBLIC," Assiut University Bulletin for Environmental Research, vol. 8, no. 1, pp. 1-16. 2005.
[6] F. Bux, and H. C. A. Kasan, "Microbiological survey of ten activated sludge plants," Water SA, vol. 20, no. 1, pp. 61-72. 1994.
[7] G.S. de Hoog, J.F. Guarro, and M. J. Gene, "Atlas of Clinical Fungi," 2nd ed. Centraalbureau voor Schimmelcultures, Utrecht, The Netherlands and Universitat Rovira i Virgili, Reus, Spain, ISBN: 978- 9070351434, pp. 1124. 2000
[8] K. H. Domsch, W. Gams, and T. Anderson, "Compendium of Soil Fungi," Lubrecht & Cramer Ltd (London). ISBN: 978-3980308380, pp. 600. 1980.
[9] D. H. Larone, "Medically Important Fungi, A Guide to Identification," 3rd ed., ASM Press, Washington, D.C., ISBN: 978-1555810917. pp. 274. 1995.
[10] A. H. Moubasher, "Soil fungi of Qatar and other Arab Countries," The Scientific and Applied Research Centre, University of Qatar, Doha, Qatar, pp 566. 1993.
[11] J. I. Pitt, and A. D. Hocking, "Fungi and food spoilage," Springer Dordrecht Heidelberg London New York. ISBN-978-0-387-92206-5. pp. 501. 2009.
[12] T. Watanabe, "Pictorial atlas of soil and seed fungi. Morphologies of cultured fungi and key to species," .2nd, CRC, Press, Boca Raton, London, New York, Washington, D.C. pp. 48. 2002.
[13] A. I. I. Abdel-Hafez, and H. M. M. EL-Sharouny, "Seasonal fluctuations of fungi in Egyptian soil receiving city sewage effluents," Cryptogamie Mycology, vol. 8, no. 3, pp. 235-249. 1987.
[14] A. I. I. Abdel-Hafez, and H. M. M. EL-Sharouny, "The occurrence of keratoinophilic fungi in sewage sludge from Egypt," Journal of Basic Microbiology, vol. 30, no. 2, pp. 73-79. 1990.
[15] S. Ali-Shtayeh, "Keratinophilic fungi isolated from children-s sandpits in the Nablus area, West Bank of Jordan," Mycopathologia, vol. 103, pp. 141- 146. 1988.
[16] M. T. Hedayati, and M. Mirzakhani, "Survey of keratinophilic fungi in sewage sludge from wastewater treatment plants of Mazandaran, Islamic Republic of Iran," Eastern Mediterranean Health Journal, vol. 15, no. 2, pp. 451-454. 2009
[17] K. Ulfig, and M. Korcz, "Keratinophilic fungi in sewage sludge applied to devastate urban soil, A preliminary experiment," International journal of environmental health research, vol. 4, pp. 244-253. 1994.
[18] K. Ulfig, M. Terakowski, G. Plaza, and O. Kosarewicz, "Keratinolytic fungi in sewage sludge," Mycopathologia, vol. 136, pp.41-46. 1996.
[19] M. D. Richardson, and B. Elewski, Fast Facts Superficial Fungal Infections," Health Press, Oxford. 2000
[20] I. Rosas, A. Baez, M. Coutino, "Bacteriological Quality of Crops Irrigation with Wastewater in the Xochimilco plots, Mexico City, Mexico," Applid and Environ Microbio, May, pp. 1074-1079. 1984.
[21] P. A. Thomas, "Current perspectives on ophthalmic mycoses," Clinical Microbiology Reviews, vol. 16, no. 4, pp. 730-797. 2003.
[22] P. Onsberg, "Human infections with Microsporum gypseum in Denmark," British Journal of Dermatology, vol. 99, no. 5, pp. 527-530. 1978.