International Science Index


Evaluation of SSR Markers Associated with High Oleic Acid in Sunflower


Sunflower oil with high oleic acid content is most desirable because of its high oxidative stability. Screening sunflower of high oleic acid using conventional method is laborious and time consuming. Therefore, the use of molecular markers as a screening tool is promising. The objective of this research was to evaluate SSR primers for high oleic acid content in sunflower. Two sunflower lines, 5A and PI 649855 were used as the representative of low and high oleic acid sunflowers, respectively, and thirty seven SSR markers were used to identify oleic acid content trait. The results revealing 10 SSR primers showed polymorphic between high and low oleic acid lines and thus were informative. With these primers, therefore, it is possible to identify the genetic markers associated with high oleic acid trait in sunflower genotypes. 

[1] J. F. Miller, D. C. Zimmerman, B. A. Vick, and W. W. Roath, “Registration of sixteen high oleic sunflower germplasm lines and bulk population,” Crop Sci, vol. 27, pp. 1323, 1987.
[2] J. Fernandez-Martinez, A. Dominguez-Gimenez, and A. Jimenez-Ramirez, “Breeding for high content of oleic acid in sunflower (Helianthus annuus L.) oil,” Helia, vol. 11, pp. 11-15, 1988.
[3] A. Pérez-Vich, L. Velasco, and J. Fernández-Martínez, “Determination of seed oil content and fatty acid composition in sunflower through the analysis of intact seeds, husked seeds, meal and oil by nearinfrared reflectance spectroscopy,” J. Am. Oil Chem. Soc, vol. 75, pp. 547-555, 1998.
[4] T. K. Nagarathna, Y. G. Shadakshari, and T. M. Ramanappa, “Molecular analysis of sunflower (Helianthus annuus L.) genotypes for high oleic acid using microsatellite markers,” Helia, vol. 34, pp. 63-68, 2011.
[5] G.N. Grandon, V. Moreno, C. Scorcione, O.J. Gieco, D. Alvarez, N. Paniego, and R. Heinz, “Characterization on sunflower inbred lines (Helianthus annuus L.) for high oleic acid content using SSR markers,” INTA, 2012.
[6] AOAC, “Official Methods of Analysis,” 16th ed. The Association of Official Analytical Chemists. AOAC, International Arlington, Verginia, USA. 1995.
[7] N. Izquierdo, L. Aguirrezabal, F. Andrade, and V. Pereyra, “Night temperature affects fatty acid composition in sunflower oil depending on the hybrid and the phonological stage,” Field Crop Res, vol. 77, pp. 115-126, 2002.
[8] S.O. Rogers, and A.J. Bendich, “Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues,” Plant MolBiol, vol. 5, pp. 69-76, 1985.
[9] A. Iqbal, H.A. Sadaqat, A.S. Khan, and M. Amjad, “Identification of sunflower (Helianthus annuus, Asteraceae) hybrids using simple-sequence repeat markers,” Genet Mol Res, vol. 10, pp. 102-106, 2010.
[10] R. Darvishzadeh, M. Azizi, H. Hatami-Maleki, I. Bernousi, B. Abdollahi Mandoulakani, M. Jafari, and A. Sarrafi, “Molecular characterization and similarity relationships among sunflower (Helianthus annuus L.) inbred lines using some mapped simple sequence repeats,” Afr J Biotechnol, vol. 9, pp. 7280-7288, October 2010.